Publications

Google Scholar

2025

Kuang D, Romand S, Zvereva AS, Orlando Marchesano BM, Grenzi M, Buratti S, Yang Q, Zheng K, Valadorou D, Mylle E, Benedikty Z, Trtílek M, Tenje M, Spetea C, Van Damme D, Wurzinger B, Schwarzländer M, Teige M, Costa A, Stael S. The burning glass effect of water droplets triggers a high light-induced calcium response in the chloroplast stroma. Curr Biol. 2025 Jun 9;35(11):2642-2658.e7.

Qi S, Mazumdar S, Zou Y, Yang Q, Stael S, James M. Four guidelines to identify bona fide protease-substrate pairs. J Exp Bot. 2025 May 9:eraf194.

Salguero-Linares J, Armengot L, Ayet J, Ruiz-Solaní N, Saile SC, Salas-Gómez M, Fernandez E, Denolf L, Navarrete F, Krumbach J, Kaiser M, Stael S, Van Breusegem F, Gevaert K, Kaschani F, Petersen M, El Kasmi F, Valls M, Coll NS, ´Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability´, EMBO Rep. 2025 Mar 20.

Stael S, Lois LM, Bachmair A, ´SUMO style defense: How post-translational modification via SUMO conjugation promotes plant defense´, Dev Cell. 2025 Mar 10;60(5):666-668.

Zou Y, Ohlsson JA, Holla S, Sabljić I, Leong JX, Ballhaus F, Krebs M, Schumacher K, Moschou PN, Stael S, Üstün S, Dagdas Y, Bozhkov PV, Minina EA, ´ATG8 delipidation is not universally critical for autophagy in plants´, Nat Commun. 2025 Jan 5;16(1):403.

2024

Cosenza-Contreras M, Seredynska A, Vogele D, Pinter N, Brombacher E, Cueto RF, Dinh TJ, Bernhard P, Rogg M, Liu J, Willems P, Stael S, Huesgen PF, Kuehn EW, Kreutz C, Schell C, Schilling O, ´TermineR: Extracting information on endogenous proteolytic processing from shotgun proteomics data´, Proteomics. 2024 Oct;24(19):e2300491.

Liu C, Hatzianestis IH, Pfirrmann T, Reza SH, Minina EA, Moazzami A, Stael S, Gutierrez-Beltran E, Pitsili E, Dörmann P, D’Andrea S, Gevaert K, Romero-Campero F, Ding P, Nowack MK, Van Breusegem F, Jones JDG, Bozhkov PV, Moschou PN,´ Seed longevity is controlled by metacaspases´, Nat Commun. 2024 Aug 8;15(1):6748.

Zou Y, Sabljić I, Horbach N, Dauphinee AN, Åsman A, Sancho Temino L, Minina EA, Drag M, Stael S, Poreba M, Ståhlberg J, Bozhkov PV (2023) ´Thermoprotection by a cell membrane-localized metacaspase in a green alga´, Plant Cell. 2024 Nov 16:koad289.

2023

Stael S, Sabljić I, Audenaert D, Andersson T, Tsiatsiani L, Kumpf RP, Vidal-Albalat A, Lindgren C, Vercammen D, Jacques S, Nguyen L, Njo M, Fernández-Fernández ÁD, Beunens T, Timmerman E, Gevaert K, Van Montagu M, Ståhlberg J, Bozhkov PV, Linusson A, Beeckman T, Van Breusegem F (2023) ´Structure-function study of a Ca2+-independent metacaspase involved in lateral root emergence´, Proc Natl Acad Sci U S A. 2023 May 30; 120(22):e2303480120.

Fernández-Fernández ÁD, Stael S, Van Breusegem F (2023) ´Mechanisms controlling plant proteases and their substrates´, Cell Death Differ. 2023 Apr;30(4):1047-1058.

Phukan UJ, Stael S, Gonçalves A, Van Breusegem F, Coll NS (2023) ´Detection and Quantification of Protein Aggregates in Plants´, Methods Mol Biol. 2023;2581:221-228.

2022

Štrancar V, van Midden KP, Krahn D, Morimoto K, Novinec M, Funk C, Stael S, Schofield CJ, Klemenčič M, van der Hoorn RAL (2022) ‘Activity-based probes trap early active intermediates during metacaspase activation’, iScience. 29;25(11):105247

Yao P, Vanneste S, Navazio L, Van Breusegem F, Stael S (2022), ‘Chemical perturbation of chloroplast Ca(2+) dynamics in Arabidopsis thaliana suspension cell cultures and seedlings’, Methods Mol Biol. 2494:149-158.

Stael S, Miller LP, Fernández-Fernández ÁD, Van Breusegem F (2022), ‘Detection of damage-activated metacaspase activity by Western blot in plants’, Methods Mol Biol. 2447:127-137.

2021

Christiaens F, Canher B, Lanssens F, Bisht A, Stael S, De Veylder L, Heyman J (2021), ‘Pars Pro Toto: Every Single Cell Matters.’ Front. Plant Sci. Jun 14;12:656825.

2020

Vega-Muñoz I, Duran-Flores D, Fernández-Fernández ÁD, Heyman J, Ritter A, Stael S (2020), ‘Breaking Bad News: Dynamic Molecular Mechanisms of Wound Response in Plants’, Front. Plant Sci. Dec 8;11:610445.

Liu C, Tornkvist A, Charova S, Stael S, Moschou PN (2020), ‘Proteolytic Proteoforms: Elusive Components of Hormonal Pathways?’ Trends Plant Sci. Apr;25(4):325-328.

Minina EA, Staal J, Alvarez VE, Berges JA, Berman-Frank I, Beyaert R, Bidle KD, Bornancin F, Casanova M, Cazzulo JJ, Choi CJ, Coll NS, Dixit VM, Dolinar M, Fasel N, Funk C, Gallois P, Gevaert K, Gutierrez-Beltran E, Hailfinger S, Klemenčič M, Koonin EV, Krappmann D, Linusson A , Machado MF, Madeo F, Megeney LA, Moschou PN, Mottram JC, Nyström T,  Osiewacz HD, Overall CM, Pandey KC, Ruland J, Salvesen GS, Shi Y, Smertenko A , Stael S, Ståhlberg J, Suárez MF, Thome M, Tuominen H, Van Breusegem F, van der Hoorn RAL, Vardi A, Zhivotovsky B, Lam E, Bozhkov PV (2020), ‘Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion With Caspases’. Mol Cell. Mar 5;77(5):927-929.

De Vriese K, Nguyen L, Stael S, Audenaert D, Beeckman T, Vanneste S (2020), ‘The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.’ Methods Mol Biol. 2213:89-98.

2019

Yao P, Deng R, Huang Y, Stael S, Shi J, Shi G, Lv B, Li Q, Dong Q, Wu Q, Li C, Chen H, Zhao H. (2019), ‘Diverse biological effects of glycosyltransferase genes from Tartary buckwheat.’ BMC Plant Biol. 19(1):339.

Stael S, Van Breusegem F, Gevaert K, Nowack MK (2019), Editorial: ‘Plant proteases and programmed cell death.’ J Exp Bot.70(7):1991-1995.

Stael S (2019), ‘Chloroplast calcium signaling quenches a thirst’, Nature Plants 5(6):559-560.

Hander T, Fernández-Fernández ÁD, Kumpf RP, Willems P, Schatowitz H, Rombaut D, Staes A, Nolf J, Pottie R, Yao P, Gonçalves A, Pavie B, Boller T, Gevaert K, Van Breusegem F, Bartels S, Stael S (2019), ‘Damage on plants activates Ca(2+)-dependent metacaspases for release of immunomodulatory peptides’, Science 22;363(6433). 

Fernández-Fernández ÁD, van der Hoorn RAL, Gevaert K, Van Breusegem F, Stael S (2019), ‘Caught green-handed: methods for in vivo detection and visualization of protease activity.’ J Exp Bot.  70(7):2125-2141.

Escamez S^#, Stael S^#, Vainonen J^#, Willems P, Jin H, Kimura S, Van Breusegem F, Gevaert K, Wrzaczek M, Tuominen H (2019), ‘Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis’, J Exp Bot. 12;70(7):2199-2210. ^#Shared first authors

2018

Bollhöner B, Jokipii-Lukkari S, Bygdell J, Stael S, Adriasola M, Muñiz L, Van Breusegem F, Ezcurra I, Wingsle G, Tuominen H (2018), ‘The function of two type II metacaspases in woody tissues of Populus trees’, New Phytol. 217(4):1551-1565.

2017

Willems P, Ndah E, Jonckheere V, Stael S, Sticker A, Martens L, Van Breusegem F, Gevaert K, Van Damme P (2017), ‘N-terminal Proteomics Assisted Profiling of the Unexplored Translation Initiation Landscape in Arabidopsis thaliana’, Mol Cell Proteomics. 16(6):1064-1080.

2016

Willems P, Mhamdi A, Stael S, Storme V, Kerchev P, Noctor G, Gevaert K, Van Breusegem F (2016), ‘The ROS Wheel: Refining ROS Transcriptional Footprints’, Plant Physiol. 171(3):1720-33.

2015

Stael S, Kmiecik P, Willems P, Van Der Kelen K, Coll NS, Teige M, Van Breusegem F (2015), ‘Plant innate immunity–sunny side up?’, Trends Plant Sci.20(1):3-11.

Wrzaczek M, Vainonen JP, Stael S, Tsiatsiani L, Help-Rinta-Rahko H, Gauthier A, Kaufholdt D, Bollhöner B, Lamminmäki A, Staes A, Gevaert K, Tuominen H, Van Breusegem F, Helariutta Y, Kangasjärvi J (2015), ‘GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis’, THE EMBO JOURNAL. 34(1):55-66.

2014

Bayer RG, Köstler T, Jain A, Stael S, Ebersberger I, Teige M (2014), ‘Higher plant proteins of cyanobacterial origin: are they or are they not preferentially targeted to chloroplasts?’, Mol Plant.7(12):1797-800.

Stael S, Nowack MK, Breusegem F, Valls M, Coll NS (2014), Editorial: ‘The death of plant cells: from proteases to field applications’, Cell Death Differ. 21(7):1178-9.

Minina A, Stael S, Van Breusegem F, Bozhkov P (2014) ‘Plant metacaspase activation and activity’, Methods Mol Biol. 1133:255-61.

Tsiatsiani L, Stael S, Van Damme P, Van Breusegem F, Gevaert K (2014) ‘Preparation of Arabidopsis thaliana seedling proteomes for identifying metacaspase substrates by N-terminal COFRADIC’, Methods Mol Biol. 1133:255-61.

Rocha AG, Mehlmer N, Stael S, Mair A, Parvin N, Chigri F, Teige M, Vothknecht UC (2014) ‘Phosphorylation of Arabidopsis transketolase at Ser428 provides a potential paradigm for the metabolic control of chloroplast carbon metabolism’, Biochem J. 458(2):313-22.

2013

Tsiatsiani L, Timmerman E, De Bock PJ, Vercammen D, Stael S, van de Cotte B, Staes A, Goethals M, Beunens T, Van Damme P, Gevaert K, Van Breusegem F (2013) ‘The Arabidopsis metacaspase9 degradome’, Plant Cell. 25(8):2831-47.

Bollhöner B, Zhang B, Stael S, Denancé N, Overmyer K, Goffner D, Van Breusegem F, Tuominen H (2013) ‘Post mortem function of AtMC9 in xylem vessel elements’, New Phytol. 200(2):498-510.

2012

Bayer RG, Stael S, Rocha AG, Mair A, Vothknecht UC, Teige M (2012) ‘Chloroplast-localized protein kinases: a step forward towards a complete inventory’, J Exp Bot. 63(4):1713-23.

Stael S, Wurzinger B, Mair A, Mehlmer N, Vothknecht UC, Teige M (2012) ‘Plant organellar calcium signalling: an emerging field’, J Exp Bot. 63(4):1525-42.

Stael S, Rocha AG, Wimberger T, Anrather D, Vothknecht UC, Teige M (2012) ‘Cross-talk between calcium signalling and protein phosphorylation at the thylakoid’, J Exp Bot. 63(4):1725-33.

2011

Stael S, Rocha AG, Robinson AJ, Kmiecik P, Vothknecht UC, Teige M (2011) ‘Arabidopsis calcium-binding mitochondrial carrier proteins as potential facilitators of mitochondrial ATP-import and plastid SAM-import’, FEBS Lett. 585(24):3935-40.

Bayer RG, Stael S, Csaszar E, Teige M (2011) ‘Mining the soluble chloroplast proteome by affinity chromatography’, Proteomics 11(7):1287-99.

Stael S, Bayer RG, Mehlmer N, Teige M (2011) Protein N-acylation overrides differing targeting signals’, FEBS Lett. 585(3):517-522.

2010

Mehlmer N, Wurzinger B, Stael S, Hofmann-Rodrigues D, Csaszar E, Pfister B, Bayer R, Teige M (2010) ‘The Ca(2+) -dependent protein kinase CPK3 is required for MAPK-independent salt-stress acclimation in Arabidopsis’, Plant J. 63(3):484-98.

Vainonen JP, Sakuragi Y, Stael S, Tikkanen M, Allahverdiyeva Y, Paakkarinen V, Aro E, Suorsa M, Scheller HV, Vener AV, Aro EM (2008) ‘Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana‘, FEBS J. 275(8):1767-77.